| 1965 Qualifications Results | |||
| Place | Team | Time | |
| 1 | Beta Theta Pi | 2:33.43 | |
| 2 | Sigma Alpha Epsilon | 2:34.25 | |
| 3 | Phi Gamma Delta | 2:36.38 | |
| 4 | Phi Kappa Psi | 2:36.40 | |
| 5 | Sigma Phi Epsilon | 2:37.00 | |
| 6 | Phi Delta Theta | 2:40.40 | |
| 7 | Kappa Delta Rho | 2:40.68 | |
| 8 | Theta Chi | 2:41.60 | |
| 9 | Lambda Chi Alpha | 2:42.00 | |
| 10 | Todd House | 2:42.78 | |
| 11 | Walnut & Laurel Halls | 2:43.?? | |
| 12 | Acacia | 2:43.48 | |
| 13 | Delta Tau Delta | 2:43.82 | |
| 14 | Jenkins-Reuter | 2:44.10 | |
| 15 | Pi Kappa Alpha | 2:44.40 | |
| 16 | Dodds House | 2:45.23 | |
| 17 | Wissler A-2 | 2:45.78 | |
| 18 | Martin III | 2:46.00 | |
| 19 | Sigma Nu | 2:46.38 | |
| 20 | Alpha Tau Omega | 2:47.36 | |
| 21 | Willkie | 2:47.68 | |
| 22 | Tau Kappa Epsilon | 2:48.00 | |
| 23 | Delta Upsilon | 2:48.50 | |
| 24 | Phi Kappa Theta | 2:49.25 | |
| 25 | Lowe & Elliott | 2:50.00 | |
| 26 | Phi Sigma Kappa | 2:51.75 | |
| 27 | Zeta Beta Tau | 2:51.80 | |
| 28 | Kappa Sigma | 2:52.48 | |
| 29 | Hummer House | 2:53.00 | |
| 30 | Alpha Epsilon Pi | 2:53.00 | |
| 31 | Jenkinson Hall | 2:53.00 | |
| 32 | Ferguson | 2:53.80 | |
| 33 | McNutt Suburbia | 2:54.89 | |